The regeneration abilities of the Greek olive varieties Kalamata (difficult to root) and Koroneiki (easy to root) have been measured in vitro using single-knot segments of plant material taken from 18-year-old trees as explants: Recently taken strong shoots cut plants and new growth after incubation of sucker shoots for both varieties; new growth after incubation of canopy shoots from Koroneiki.
The regeneration ability was evaluated by measuring the dry weight increase of leaves and cultures and the start of roots in explants. Explants from both varieties did not produce significantly different numbers of shoots. However, explants from two shoots of strong Koroneii produced the longest total shoot lengths and the largest number of leaves on new shoots, both of which are statistically significant. Significant increases in explant dry weights were found only in explants taken from vigorous shoots of both varieties. Root initials were produced in vitro 40 days after inoculation at the rates of 29 (Kalamata) and 45 (Koroneiki), but only from explants obtained from strong shoots; other explants do not produce root initials. From the sucker and canopy shoots of Kalamata, stored in water and in the dark, he produced cuttings, also roots, to encourage them to produce new etiolated shoots: 41 for suckers and 13 for canopy shoots. Similar cuttings of Koroneiki did not produce roots. Attempts have been made to promote somatic embryogenesis in the callus produced by vigorous shoots of both varieties, as well as adventurous shoot formation in root cuttings, but these have not been successful. Root initials were produced in vitro 40 days after inoculation at the rates of 29 (Kalamata) and 45 (Koroneiki), but only from explants obtained from strong shoots; other explants do not produce root initials. From the sucker and canopy shoots of Kalamata, stored in water and in the dark, he produced cuttings, also roots, to encourage them to produce new etiolated shoots: 41 for suckers and 13 for canopy shoots. Similar cuttings of Koroneiki did not produce roots. Attempts have been made to promote somatic embryogenesis in the callus produced by vigorous shoots of both varieties, as well as adventurous shoot formation in root cuttings, but these have not been successful. Root initials were produced in vitro 40 days after inoculation at the rates of 29 (Kalamata) and 45 (Koroneiki), but only from explants obtained from strong shoots; other explants do not produce root initials. From the sucker and canopy shoots of Kalamata, stored in water and in the dark, he produced cuttings, also roots, to encourage them to produce new etiolated shoots: 41 for suckers and 13 for canopy shoots. Similar cuttings of Koroneiki did not produce roots. Attempts have been made to promote somatic embryogenesis in the callus produced by vigorous shoots of both varieties, as well as adventurous shoot formation in root cuttings, but these have not been successful. From the sucker and canopy shoots of Kalamata, stored in water and in the dark, he produced cuttings, also roots, to encourage them to produce new etiolated shoots: 41 for suckers and 13 for canopy shoots. Similar cuttings of Koroneiki did not produce roots. Attempts have been made to promote somatic embryogenesis in the callus produced by vigorous shoots of both varieties, as well as adventurous shoot formation in root cuttings, but these have not been successful. From the sucker and canopy shoots of Kalamata, stored in water and in the dark, he produced cuttings, also roots, to encourage them to produce new etiolated shoots: 41 for suckers and 13 for canopy shoots. Similar cuttings of Koroneiki did not produce roots. Attempts have been made to promote somatic embryogenesis in the callus produced by vigorous shoots of both varieties, as well as accidental shoot formation in root cuttings, but these have not been successful.
Comments